Description This General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane to which the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications. Applications Real-time-PCR (RT-PCR) Real-time quantitative Northern blotting Nuclease protection assays RNA amplification for microarray analysis cDNA library preparation after poly(A)+ selection Feature •Stable yield •Reliable performance of high-quality purified total RNA in downstream applications Store Store at 2-8℃, protect from light. Kit contents are stable for up to 12 months, when properly stored.

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